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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Dev Dyn. 2017 Feb;246(2):135–147. doi: 10.1002/dvdy.24476

Figure 5. Inhibition of RA signaling upregulates expression of Krt5 and Krt14 mRNA.

Figure 5

Quantitation of gene expression by qPCR demonstrates that Krt5 and its dimerization partner Krt14 are upregulated by inhibition of RA signaling with BMS 493. Krt5 is upregulated ≥ 24 fold (p = 0.000002) and Krt14 is upregulated ≥ 8 fold (p ≤ 0.03) in ER grown in BMS 493 relative to controls. No significant change in expression is observed for keratins Krt8 or Krt19. Data represent averages for 3 independent culture experiments with 6-8 ER/condition. For Krt5 each cDNA sample was run as 6 technical qPCR replicates, for all others each sample was run as 3 technical replicates. Error bars represent standard deviations.