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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Atherosclerosis. 2016 Dec 15;257:38–46. doi: 10.1016/j.atherosclerosis.2016.12.014

Fig. 5. Expression of P2Y2 receptor prevents smooth muscle cell calcification in vitro.

Fig. 5

(A) Wild-type (WT) and P2Y2R−/− VSMC were incubated in high phosphate (High Pi) or control medium (Low Pi) for 7 days and stained with alizarin red. Scale bar =100μm. (B) Calcium deposition in VSMC was quantified as in Fig. 4. Fold changes in the mRNA expression of osteogenic markers Runx2 and osteocalcin relative to that seen in WT cells are shown in bar graphs. Results are presented as mean ± SEM. ***p < 0.001; n=4 independent experiments. Transduction of WT P2Y2R into P2Y2R−/− inhibits (C) calcium deposition as well as (D) relative mRNA levels for Runx2 and osteocalcin. ***p < 0.001; n=3 independent experiments; NS= not significant.