Table 2. Silencing BRAF expression reduces cell death enhancement induced by differentiation agent combination in HL60 and U937 cells.

A		TB exclusion	P value	P value	Annexin V assay
	HL60 cells	Cell death-Mean	vs CTL	vs AraC	Apoptosis	Necrosis	Total death
	1. siControl-Control	7.5%			4.9%	4.8%	9.7%
	2. siControl-D2/CA	12.8%	0.009		5.8%	6.4%	12.2%
	3. siControl-AraC	33.0%	0.003		12.0%	13.5%	25.5%
	4. siControl-AraC-D2/CA	47.3%	0.001	0.001	15.0%	18.9%	33.9%
	5. siBRAF-Control	11.0%			6.2%	6.5%	12.7%
	6. siBRAF-D2/CA	12.5%	0.339		5.9%	6.5%	12.3%
	7. siBRAF-AraC	29.0%	0.007		10.5%	10.1%	20.6%
	8. siBRAF-AraC-D2/CA	39.0%	0.003	0.009	12.4%	14.1%	26.4%
B		TB exclusion	P value	P value	Annexin V assay
	U937 cells	Cell death-Mean	vs CTL	vs AraC	Apoptosis	Necrosis	Total death
	1. siControl-Control	9.0%			7.8%	4.6%	12.4%
	2. siControl-D2/CA	10.3%	0.691		10.1%	6.0%	16.1%
	3. siControl-AraC	49.7%	0.006		26.7%	14.1%	40.8%
	4. siControl-AraC-D2/CA	58.3%	0.009	0.049	36.6%	25.6%	62.2%
	5. siBRAF-Control	10.7%			7.5%	5.5%	13.0%
	6. siBRAF-D2/CA	11.3%	0.529		8.8%	7.0%	15.8%
	7. siBRAF-AraC	47.7%	0.001		26.5%	13.2%	39.7%
	8. siBRAF-AraC-D2/CA	54.0%	0.004	0.092	33.2%	21.1%	54.3%

Experimental details are described in Materials and methods and the legend for Fig 1.