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Phosphorylation of PDGFR at 5 different sites was investigated in HASMC lysates upon Sema3A±PDGF stimulation at different time points by using specific antibodies. Total PDGFR was used as a loading control. PDGF stimulation robustly induced phosphorylation of its receptor while Sema3A alone did not affect PDGFR phosphorylation. Sema3A and PDGF co-stimulation reduced PDGF-induced phosphorylation of PDGFR at different time points.
