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. 2016 Oct 26;7(49):80262–80274. doi: 10.18632/oncotarget.12918

Figure 6. Andrographolide inhibited LPS-induced NO, IL-6 and TNF-α production in macrophages.

Figure 6

Raw264.7 cells were treated with various concentrations of Andrographolide in the absence or presence of LPS. A. The survival rate of 24 h was determined by MTT assay in the presence of Andrographolide. The levels of NO, IL-6 and TNF-α in the cell culture medium were determined 24 h after LPS stimulation as described in Methods. B. mRNA levels of iNOS, COX2, IL-6 and TNF-α in cells were examined 6 h after LPS stimulation by RT-PCR. Values were shown as the means ± SEM of three independent experiment. *P < 0.05, **P < 0.01 vs. LPS group.