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. 2016 Nov 10;7(49):80275–80287. doi: 10.18632/oncotarget.13282

Figure 2. The ALV-J capsid protein p27 promotes IL-6 expression in a dose-dependent manner in splenocytes.

Figure 2

A. The expression of p27, gp85, integrase, reverse transcriptase, and gp37 were confirmed by western blot. Splenocytes were transfected with the pCAGGS vector or ALV-J p27, gp85, gp37, reverse transcriptase, or integrase expression vectors for 48 h. Then, IL6 mRNA levels were determined using real-time RT-PCR B. and IL-6 protein levels were determined using ELISA C.