Table 1. Sequences of oligos and assays ID used for in vitro experiments.
Oligos | Sequence |
---|---|
For cloning | |
Hs_SMARCA F | AGTAGATCTTCAGGCTATGGACC |
Hs_SMARCAR | ACTTCTTCTGGAGTTTTGCCTTC |
For mutagenesis | |
Hs_SMARCA_MUT-F | T ATTTC AGGGA AGCTCTTCGTGTT AGTGA A ATGTTC AGGATTTCC AGTTCTTTCCTCC AC |
Hs_SM ARCA_MUT-R | GTGGAGGAAAGAACTGGAAATCCTGAACATTTCACTAACACGAAGAGCTTCCCTGAAATA |
For qPCR | |
HS_SMARCA5_RT_F | TCTGTTGCCAGATGTGTTTAATTCA |
HS_SMARCA5_RT_R | CCCAAGGCAGTTGTTTGTATCA |
MIMIC and INHIBITOR | Commercial code |
mimic hsa-miR-151-5p | SIGMA HMI0244 |
inhibitor hsa-miR-151 -5p | SIGMA HSTUD0244 |
mimic control negative | SIGMA HMC0002 |
inhibitor control negative | SIGMA NCSTUD001 |