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. 2016 Nov 2;7(49):80612–80632. doi: 10.18632/oncotarget.13016

Figure 4. Pygo2 ablation downregulates different Wnt/ß-catenin target genes overexpressed during intestinal hyperproliferation induced by Apc LOF and Ctnnb1 GOF.

Figure 4

(A, B) Representative Immunostains with the indicated antibodies on intestinal tissue sections from compound homozygous Apc LOF mice (A) and heterozygous Ctnnb1 GOF animals (B). The upper panel shows the stainings from controls. Pictures from immunostains of compound mutant animals with wild type Pygo2 expression and hetero- and homozygous Pygo2 ablation are provided below. Scale bars in the pictures represent 200 μm for all IHC. Inserts show the staining at higher magnification. (C) Relative RNA expression of Wnt/ß-catenin target genes in Apc LOF and Ctnnb1 GOF mutant animals. qRT-PCR was performed with RNA extracted from intestinal epithelial cells of compound mutant animals as indicated below the graphs. Significances are marked with *P < 0.05, and **P < 0.01 for the mean expression level compared to the respective control group.