Figure 6. Knockout of Pygo2 downregulates overexpressed Wnt/ß-catenin targets and tumor progression genes in a subgroup of Pygo2 deficient chemically induced intestinal tumors.

(A) Representative immunohistochemical stainings of chemically induced intestinal tumors for the indicated markers. Tissues from controls (Pygo2^{lox(ex3)/lox(ex3)}) and Pygo2 knockout animals (Vil^Cre; Pygo2^Δ/Δ) were collected six months after treatment with AOM and DSS. Scale bars in the pictures represent 200 μm for all IHC. Inserts show the staining at higher magnification. (B) qRT-PCR analyses of RNA extracted from tumors and from colon tissues of untreated mice, both of controls and Pygo2 deficient animals. Each graph shows the relative RNA expression of the indicated gene for one single animal. Significances were calculated for the mean expression level compared to untreated controls and relative to the expression levels of control tumors, respectively. ** marks significant differences between the respective groups with P < 0.01.