Table 1. Summary for the relative regulation of target gene expression in the tumor models with and without knockout of Pygo2.

tumor model	AOM+DSS tumors		Apc LOF		Ctnnb1 GOF
genotype	Pygo2 WT	Pygo2 KO	Pygo2 WT	Pygo2 KO	Pygo2 WT	Pygo2 KO
Wnt/ß-catenin signaling co-factors
Pygo2	↑	↓↓↓	↑	↓↓↓	↑↑	↓↓↓
Pygo1	n.e.	n.e.	n.e.	n.e.	n.e.	n.e.
Bcl9	≈	≈	≈	≈	≈	≈
Bcl9-2	↑↑	↑↑	≈	≈	(↑)	≈
Classical Wnt target genes
Axin2	↑↑↑	↑	↑↑	↑	↑↑	≈
CyclinD1	↑↑	↑↑	↑↑	↑↑	↑	≈
Tcf1	↑↑↑	↑↑	↑↑↑	↑↑	↑↑↑	↑↑
Tcf4	≈	↓	≈	↓	↑	↓
Lef1	↑↑↑	↑	↑↑↑	↑	↑↑↑	↑
Stem cell marker
Ascl2	↑↑↑	↑↑	↑↑↑	↑↑	↑↑	↑
Lgr5	↑↑↑	↑↑	↑↑↑	↑↑	↑	≈
Dclk1	↓	↓	↓	↓	≈	≈
Tumor progression marker
Sox9	↑↑↑	↑↑	↑↑↑	↑↑↑	↑↑	↑
Prox1	↑↑↑	↑↑	↑↑	≈	↑↑↑	≈
c-myc	↑↑	↑↑	↑↑↑	↑↑↑	↑ / ↑↑	≈

Relative expression level of target genes analyzed in this study. The summary is based on RNA expression as determined by qRT-PCR analyses and protein expression as detected by IHC. The increase ↑ or decrease ↓ is indicated relative to the respective uninduced controls in normal intestinal tissues. ≈ marks unchanged expression compared to control. Shown are the results from chemically induced tumors (AOM+DSS tumors) on Pygo2 wild type background and the subgroup of tumors with reduced expression following Pygo2 ablation (left column). The middle and right columns summarize the relative expression in homozygous Apc LOF and heterozygous Ctnnb1 GOF mutants with Pygo2 wild type expression compared to Pygo2 knockout animals.