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. 2016 Oct 13;7(49):80765–80782. doi: 10.18632/oncotarget.12623

Figure 1. Knockdown of Hmgb1 expression decreases invasion in melanoma cells.

Figure 1

A. Western blot of Klotho and Hmgb1 protein expression in WM35, WM451, SK-28, A375 cells. Cells were transfected with or without pGFP-shHmgb1 and treated with or without LPS. NC: negative control. Cells were treated with transfection reagents. BC: blank control. Cells were transfected with blank vector. shHmgb1: cells were transfected with pGFP-shHmgb1 vector to express Hmgb1 shRNA. shHmgb1+LPS: cells were transfected with pGFP-shHmgb1 vector to express Hmgb1 shRNA and treated with LPS (10 ng/ml). LPS: cells were only treated with LPS (10 ng/ml). B. Evidence of the efficacy of Hmgb1 knockdown in A375 cells. C. Evidence of the efficacy of Hmgb1 knockdown in SK-28 cells. D. Representative photographs of cell invasion in A375and SK-28 cells. LPS: LPS alone. E. The number of cell invasion in A375 cells. F. The number of cell invasion in SK-28 cells. *P<0.001 between two groups. N=4.