Skip to main content
. 2016 Nov 4;7(49):80820–80841. doi: 10.18632/oncotarget.13098

Figure 10. TRAIL-induced de-phosphorylation of TrkAIII Y674/675 associates with apoptosis and is prevented by PP1 and NSC-87877.

Figure 10

(A) Western blots demonstrating no change total and Y674/675 phosphorylated TrkAIII levels in TrkAIII SH-SY5Y cells incubated for 0, 3 and 6 hours in absence of TRAIL, compared to time-dependent TRAIL-induced (200 ng/ml) TrkAIII Y674/675 de-phosphorylation associated with constant TrkAIII levels, in TrkAIII SH-SY5Y cells incubated for 3 and 6 hours plus the inhibition of TRAIL-induced TrkAIII Y674/675 de-phosphorylation by 1 μM PP1 (TRAIL + PPI) and by 1 μM NSC-87877 (TRAIL + NSC) at both 3 and 6 hours. (B) Histograms demonstrating the mean (±SD) change the ratio of total to Y674/675-phosphorylated TrkAIII in whole cell extracts (20 μg) from TrkAIII SH-SY5Y cells incubated for 0–6 hours in the absence of TRAIL (control) in the presence of TRAIL (200 ng/ml) alone (TRAIL), with TRAIL plus 1 μM PP1 (TRAIL + PP1) or with TRAIL plus 1 μM NSC-87877 (TRAIL + NSC), assessed by ImageJ densitometry of 3 independent Western blots (* = significant difference compared to untreated 6 hour controls).