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. 2016 Nov 4;7(49):80842–80854. doi: 10.18632/oncotarget.13099

Figure 3. Oxaliplatin induced mTOR-dependent autophagy is required for CRT surface exposure.

Figure 3

(A–B) HCT116 Cells were treated for 8 h with Oxaliplatin (Oxa) 10 μg/ml or Lithium (Li) 10 mM and with or without TNP 20 μM, then subjected to immunoblot detection of LC3 in the total cell lysate (A) or Ecto-CRT detection (B); (C) HCT116 Cells were treated with 10 μg/ml Oxa, Oxa plus PI3K siRNA or Oxa plus 50 nM Wortmannin (Wort) for 8 h, then subjected to immunoblot detection; (D) HCT116 Cells were treated with 10 μg/ml Oxa or Oxa plus PI3K siRNA for 8 h, then subjected to immunoblot detection; (E) HCT116 Cells were treated with mTOR siRNA for 24 h or 10 μg/ml Oxa for 8 h, then subjected to immunoblot detection; (F) HCT116 cells were treated with 10 μg/ml Oxa or Rapamycin (Rapa) 100 nM for 8 h, then cells were subjected to immunoblot detection; (G left) Cells were transfected with or without PI3K siRNAs for 12 h, then maintained in media with 10 μg/ml Oxa or 50 nMWort for 8 h, then subjected to Ecto-CRT detection; (G middle) Cells were transfected with or without ULK1 siRNAs for 12 h, then maintained in media with 10 μg/ml Oxa for 8 h, then subjected to Ecto-CRT detection; (G right) Cells were transfected with or without ULK1 siRNAs for 24 h or treated with 10 μg/ml Oxa or 100 nM Rapa for 8 h, then subjected to Ecto-CRT detection. Results are representative of three independent experiments.