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. 2016 Nov 4;7(49):80842–80854. doi: 10.18632/oncotarget.13099

Figure 4. Oxaliplatin promotes Beclin 1 phosphorylation by enhancing the association with ULK1.

Figure 4

(A) HCT116 cells were treated with 10 μg/ml Oxaliplatin (Oxa) for indicated times or 50 nM Bortezomib (Bor) for 24 h, then subjected to immunoblot detection; (B) HCT116 cells were treated with 10 μg/ml Oxaliplatin (Oxa) or 100 nM Rapamycin (Rapa) for 8 h were lysed and subjected to immunoprecipitation analysis using an indicated antibody, then immunoprecipitates were subjected to immunoblot detection; (C) HCT116 cells were treated with 1 μg/ml Oxa or 250 nM Torin-1 for 8 h were lysed and subjected to immunoblot detection; (D) Cells were transfected with ULK 1 siRNAs for 12 h and maintained in media with 1 μg/ml Oxa for 8 h, then were followed by immunoblot detection. Results are representative of three independent experiments.