Skip to main content
. 2016 Oct 22;7(49):81208–81222. doi: 10.18632/oncotarget.12820

Figure 3. The enhanced cell kill observed in the BN175 cells after treatment with GLV-1h68 and EBRT is not due to increased viral replication but mediated via induction of intrinsic apoptosis and downregulation of the anti-apoptotic MCL-1 protein.

Figure 3

A. Viral plaque assay (VPA) investigating the recovery of live GLV-1h68 at progressive time points post-treatment with GLV-1h68 MOI 0.01 (VV) alone or in combination with radiation (0-8 Gy) and B. with GLV1h68 MOI 0.1. C. Western blot analysis of cleaved caspase 3 and Serine-15 phosphorylated p53 and D. Caspase-Glo assay of caspase 3/7 activity 48 hours post-combination therapy (** = p value <0.01). E. Western blot analysis of BN175 cells 48 hours after treatment with GLV-1h68 (MOI 0.1) and EBRT (4 Gy).