Figure 1. The expression patterns of USP33 in CRC and CRCLM tissues.

Representative immunostaining of USP33 expression in adjacent nontumorous colon tissue (A, high expression), primary CRC tissue (B, low expression), adjacent nontumorous liver tissue (C, high expression), and CRCLM tissue (D, low expression) by IHC analysis. Fresh-frozen specimens from 14 patients showed that mRNA level of USP33 in CRC tissue was lower than that of adjacent nontumorous colon tissue (E). Similarly, the USP33 mRNA level in CRCLM tissue was significantly lower compared to adjacent liver tissue (F). The protein level of USP33 in fresh-frozen tissues was also assessed by Western Blot (G). (scale bar: 100 μM)