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. 2016 Nov 9;7(49):81367–81376. doi: 10.18632/oncotarget.13229

Figure 5. miR-26a could directly binding with KRT19 3′UTR.

Figure 5

The protein expression levels of KRT19 were detected by western blot in cells treated with miR-26a mimics, inhibitor or controls (A) RBE cell line, (B) HCCC-9810 cell line. (C) The sequence alignment of human miR-26a with 3′ UTR of KRT19. Bottom: mutations in the 3′-UTR of KRT19 in order to create the mutant luciferase reporter construct. (D) Cells were co-transfected with miR-26a mimics or Control, Renilla luciferase vector pGL3-SV40 and KRT19 3′UTR luciferase reporters for 48 h. Both firefly and Renilla luciferase activities were measured in the same sample. Firefly luciferase signals were normalized with Renilla luciferase signals. **indicated remarkable significant difference (P < 0.01). All tests were performed in triplicate and presented as mean ± SE.