Skip to main content
. 2016 Nov 10;7(49):81452–81462. doi: 10.18632/oncotarget.13263

Figure 2. LncRNA H19 contributed to multidrug resistance in breast cancer cells.

Figure 2

(A) MCF-7R or ZR-75-1R cells were transiently transfected with H19-targeting siRNA or scrambled siRNA. Real-time PCR analysis showed that H19 expression was effectively reduced. An MTT assay indicated that knockdown of H19 decreased cell viability under the stress of PTX treatment. (B) MCF-7S or ZR-75-1S cells were transiently transfected with H19 overexpression plasmids or control vectors. Real-time PCR analysis confirmed the overexpression of H19. An MTT assay was performed to determine cell viabilities of MCF-7S or ZR-75-1S cells treated with PTX at different concentrations. (C) MTT results obtained from MCF-7S cells transfected with H19 overexpression plasmids or control vectors showed that H19 overexpression also increased the resistance of these cells to both EPI (left) and DDP (right). (D) The MTT results obtained from MCF-7R cells transfected with H19-targeting siRNA or control siRNA showed that H19 knockdown also decreased the resistance of these cells to both EPI and DDP.