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. 2016 Nov 3;7(49):81474–81492. doi: 10.18632/oncotarget.13034

Figure 1. Human sTWEAK overexpression in murine B16 melanoma cells increases Fn14 expression.

Figure 1

A. Vector control (V1, V2) and TWEAK-myc plasmid-transfected (T1, T2) B16 clonal cell lines were harvested and cell lysate and conditioned media samples were prepared. TWEAK and GAPDH levels were analyzed by Western blotting using anti-myc tag and anti-GAPDH antibodies, respectively. This Western blot was done three times. B. Human sTWEAK levels in conditioned media samples collected from the four B16 cell lines were determined by ELISA. The results shown are the combination of 2 independent experiments for V1/T1 and 3 independent experiments for V2/T2. C. The four B16 cell lines were harvested and Fn14 and GAPDH levels were evaluated by Western blot analysis. This Western blot was done three times.