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. 2016 Nov 3;7(49):81474–81492. doi: 10.18632/oncotarget.13034

Figure 4. Both TWEAK overexpression in B16 cells and exogenous TWEAK treatment of B16 cells inhibits cell invasion.

Figure 4

A. The four B16 cell lines were evaluated for invasive capacity using the Matrigel invasion assay. The number of invaded cells for each invasion chamber was determined from photographs of eight randomly chosen fields. The values shown are the mean ± SEM for three chambers per cell line. Significance was measured by Student's t-test. B. V1 cells were either left untreated or treated with 200 ng/ml of TWEAK (TWK) for 24 hr prior to plating in Matrigel invasion chambers. T1 cell invasion was also evaluated. The values shown are the mean ± SEM for three chambers per condition. Significance was measured by Student's t-test. C. V2 cells were either left untreated or treated with 200 ng/ml of TWEAK (TWK) for 24 hr prior to plating in Matrigel invasion chambers. T2 cell invasion was also evaluated. The values shown are the mean ± SEM for three chambers per condition. Significance was measured by Student's t-test. All invasion assays were performed at least two times and the results of one representative experiment are shown here.