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. 2016 Nov 3;7(49):81474–81492. doi: 10.18632/oncotarget.13034

Figure 6. NF-κB2 p100 depletion in B16 T2 cells increases cell invasion.

Figure 6

A. V2 and T2 cells were either left untreated or T2 cells were transiently transfected with control (CTL) siRNA, p100 siRNA #3 or p100 siRNA #4 for 48 hr. Cells were harvested and NF-κB2 (p100/p52) and GAPDH levels were evaluated by Western blot analysis. This Western blot was done twice. B. V2 and T2 cells were either left untreated or T2 cells were transiently transfected with control (CTL) siRNA, p100 siRNA #3 or p100 siRNA #4 for 24 hr prior to cell harvest and plating in Matrigel invasion chambers. The number of invaded cells for each invasion chamber was determined from photographs of eight randomly chosen fields. The values shown are the mean ± SEM for three chambers per condition, with each chamber done independently. Significance was measured by Student's t-test (ns = not significant; p > 0.05).