Figure 7. Navitoclax-induced cell viability inhibition and apoptosis can be reversed by pretreatment of Z-VAD-FMK in NCI-H1975/OSIR cells.

A. NCI-H975/OSIR cells were treated with navitoclax for 48 h with or without pretreatment of Z-VAD-FMK (10 μM, 1 h). Cell viability was then evaluated using MTT assay. *P<0.05 and **P<0.01. B. After pretreatment with 10 μM Z-VAD-FMK for 1 h, NCI-H1975/OSIR cells were incubated with 0.5 μM navitoclax for 48 h, the apoptotic cells were stained with annexin V/PI and determined by a flow cytometry according to the manufacturer's instructions. *P<0.05 and **P<0.01.