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. 2016 Nov 7;7(49):81634–81644. doi: 10.18632/oncotarget.13155

Figure 3. AEG-1 was involved in radiation-enhanced migration and invasion in colon cancer cell lines.

Figure 3

Stable AEG-1 knockdown and negative control cells of the SW480 and HCT116 cell lines were seeded in FBS containing medium in migration chamber or in matrigel invasion chamber respectively, placed in 24 well plates with FBS containing medium and either irradiated with 7.5 Gy or unirradiated as a control. After 24 h, migration and invasion were determined by counting the cells migrated or invaded into the membrane. A. Migration significantly increased upon radiation in both the SW480 negative control (1.56 fold; p=0.015) and SW480 AEG-1 knockdown (1.85 fold; p=0.03) cell lines. In the HCT116 cell line, migration was increased only in the negative control cell line (1.36 fold; p=0.049) whereas in the AEG-1 knockdown cell line the migration rate did not change (0.92 fold; p=0.077) B. Invasion significantly increased upon radiation in the SW480 negative control cell line (3.71 fold; p=0.039), but not in the AEG-1 knockdown cell line (1.34 fold; p=0.27). Data are presented as the ratios between 7.5 Gy and 0 Gy for each cell line. NC: negative control; AEG-1: AEG-1 knockdown. Error bars represent mean ± SD and only significant p-values are presented (p<0.05). All the experiments were performed in duplicates at least three times.