Skip to main content
. 2016 Nov 7;7(49):81634–81644. doi: 10.18632/oncotarget.13155

Figure 5. Cytokine array revealed that MMP-8, MMP-9 and EGF were decreased upon radiation in AEG-1 knockdown colon cancer cell lines.

Figure 5

Conditioned medium of negative control and AEG-1 knockdown cell lines were tested for MMP and cytokine secretion upon radiation by using an antibody array. A. MMP-8, MMP-9 and EGF were decreased in the irradiated AEG-1 knockdown cells compared to the unirradiated control, whereas there was a small increase or no change in the negative control cell line. Data presented as the ratios between the 7.5 Gy and 0 Gy for each cell line. B. MMP-9 mRNA expression analyzed by qPCR was decreased in SW480 AEG-1 knockdown cells compared to negative control cells. NC: negative control; AEG-1: AEG-1 knockdown. Error bars represent mean ± SD. All the experiments were performed at least three times.