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. 2016 Nov 7;7(49):81645–81660. doi: 10.18632/oncotarget.13162

Figure 2. AREG up-regulates SPRY2 expression in human ovarian cancer cells.

Figure 2

A. SKOV3 cells were treated with 100 ng/mL AREG for different time periods, and SPRY2 mRNA levels were examined by RT-qPCR. SPRY2 mRNA level at each time point was normalized to GAPDH mRNA level at the same time point. B. SKOV3 cells were treated for 3 h with vehicle control or different concentrations of AREG, and SPRY2 mRNA levels were examined by RT-qPCR. C. SKOV3 cells were treated with 100 ng/mL AREG (AR) for different time periods, and SPRY2 protein levels were examined by Western blot. D. SKOV3 cells were treated for 3 h with vehicle control or different concentrations of AREG, and SPRY2 protein levels were examined by Western blot. The results are presented as the mean ± SEM of at least three independent experiments. Values without a common letter are significantly different (P < 0.05).