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. 2016 Nov 7;7(49):81645–81660. doi: 10.18632/oncotarget.13162

Figure 3. EGFR is required for the AREG-induced up-regulation of SPRY2 expression.

Figure 3

A and B. SKOV3 cells were pre-treated with vehicle control (DMSO) or 10 μM AG1478 for 1 h and then treated with vehicle control (Ctrl) or 10 ng/mL AREG (AR) for 3 h. The mRNA (A) and protein (B) levels of SPRY2 were examined by RT-qPCR and Western blot, respectively. C and D. SKOV3 cells were transfected with 50 nM control siRNA (si-Ctrl) or EGFR siRNA (si-EGFR) for 48 h and then treated with vehicle control (Ctrl) or 10 ng/mL AREG (AR) for 3 h. The mRNA (A) and protein (B) levels of SPRY2 and EGFR were examined by RT-qPCR and Western blot, respectively. The results are presented as the mean ± SEM of at least three independent experiments. Values without a common letter are significantly different (P < 0.05).