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. 2016 Nov 7;7(49):81645–81660. doi: 10.18632/oncotarget.13162

Figure 7. Overexpression of SPRY2 attenuates the AREG-induced up-regulation of Snail expression.

Figure 7

A and B. SKOV3 cells were transfected for 48 h with 1 μg control vector (Vector) or SPRY2 overexpression vector (SPRY2) and then treated with 10 ng/mL AREG (AR) for 3 h. The mRNA (A) and protein (B) levels of Snail, Slug and SPRY2 were examined by RT-qPCR and Western blot, respectively. C. OVCAR5 cells were transfected for 48 h with 1 μg control vector (Vector) or SPRY2 overexpression vector (SPRY2) and then treated with 10 ng/mL AREG (AR) for 3 h. The protein levels of Snail, Slug and SPRY2 were examined by Western blot. The results are presented as the mean ± SEM of at least three independent experiments. Values without a common letter are significantly different (P < 0.05).