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. 2016 Nov 7;7(49):81645–81660. doi: 10.18632/oncotarget.13162

Figure 8. Overexpression of SPRY2 attenuates AREG-induced cell invasion and proliferation.

Figure 8

A. SKOV3 (left panel) and OVCAR5 (right panel) cells were transfected with 1 μg control vector (Vector) or SPRY2 overexpression vector (SPRY2) for 48 h. After transfection, the cells were treated with 10 ng/mL AREG, and the effects of AREG on cell invasion were examined in Matrigel invasion assays. B. SKOV3 (left panel) and OVCAR5 (right panel) cells were transfected with 1 μg control vector or SPRY2 overexpression vector (SPRY2) for 48 h. After transfection, the cells were treated with 10 ng/mL AREG every 24 h, and the number of cells was quantified using a trypan blue exclusion assay. The results are presented as the mean ± SEM of at least three independent experiments. Values without a common letter are significantly different (P < 0.05).