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. 2017 Mar 14;8:433. doi: 10.3389/fmicb.2017.00433

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Copyright © 2017 Kim, Shin, Park and Park.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Monitoring the expression of genes in response to indole concentrations in P. putida KT2440. The expression of genes involved in (A) the tryptophan pathway (trpB), (B–D) Amp resistance (ampC, ttgA, and oprD) and (E–G) DNA repair/mutagenesis (rpoS, recA, and mutS) were evaluated. Total RNA was isolated from cultures after 8 h of incubation with 100 μg/ml Amp and indole (0, 50, 100, 250, 500, and 1000 μM) or indole alone (0, 50, and 1000 μM) without Amp. All data represent the average of three replicates, and the error bar indicates the standard deviation.