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. 2017 Mar 14;10:75. doi: 10.3389/fnmol.2017.00075

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Copyright © 2017 Wang, Xu, Liu, Zhang, Gao, Yin, Jiang, Cao, Yu, Cao and Kou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hydrogen peroxide (H₂O₂) induces apoptosis and membrane blebbing in PC12 cells and neurons. (A) PC12 cells and neurons were exposed to 100 μM H₂O₂ for 12 h. Caspase-3 activity was evaluated in PC12 cells and neurons. (B) PC12 cells morphology using phase-contrast microscopy. Bar, 10 μm. (C) Transmission electron microscopy (TEM) of PC12 cells. Bar, 1 μm. (D) Neurons were imaged through differential interference contrast (DIC) using confocal laser scanning microscope (LSM). Bar, 5 μm. Results were expressed as mean ± SD from three independent experiments (^#P < 0.05 vs. control, ^##P < 0.01 vs. control).