Figure 11.

Caspase, ROCK, myosin II inhibition attenuates H₂O₂-induced signaling pathway activation in PC12 cells. PC12 cells were untreated or pretreated with 1 μM blebbistatin, 10 μM Y27632 or 10 μM z-VAD-fmk for 1 h prior to 100 μM H₂O₂ treatment for 12 h. NAC served as positive control. Total cell lysates were prepared, subjected to Western blot analysis with antibodies to caspase-3, cleaved caspase-3 (A), ROCK1, cleaved ROCK1 (B), MLC and P-MLC (Ser-19) (C), and incubated with anti-GAPDH antibody as loading control. Results were expressed as mean ± SD from three independent experiments (^##P < 0.01 vs. control, *P < 0.05 vs. H₂O₂-treated cells, **P < 0.01 vs. H₂O₂-treated cells).