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. 2017 Mar 14;8:111. doi: 10.3389/fphar.2017.00111

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Copyright © 2017 Busceti, Marchitti, Bianchi, Di Pietro, Riozzi, Stanzione, Cannella, Battaglia, Bruno, Volpe, Fornai, Nicoletti and Rubattu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TGF-β neutralizing antibody does not inhibit Smad1,5,8 phosphorylation and the VEGF upregulation induced by Dkk-3. (A) Western blot analysis of phosphoSmad1,5,8 in cells exposed for 60 min to hrDkk-3 either in the presence or in the absence of neutralizing TGF-β antibody. (B) Corresponding densitometric analysis. Values are means ± SD. (C) Western blot analysis of VEGF in cells exposed for 12 h to hrDkk-3 either in the presence or in the absence of neutralizing TGF-β antibody. (D) Corresponding densitometric analysis. Values are means ± SD.