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. 2017 Mar 14;7:83. doi: 10.3389/fcimb.2017.00083

Table 1.

Expression of heat shock, molecular chaperone, LPS O-antigen biosynthesis, and LPS phosphorylation genes in response to Tobramycin and host cells.

Gene ID Name Description Sample Name
P0.3 P2 APD0 APD0.3
Components
PAO1 PAO1 PAO1 PAO1
0.3 μg/ml Tobramycin 2 μg/ml Tobramycin No Tobramycin A549 and dHL60 0.3 μg/ml Tobramycin A549 and dHL60
Tobramycin response Fold change* P-value Fold change* P-value Fold change* P-value Fold change* P-value
PA3126 ibpA heat shock protein IbpA 0.94 6.40E-01 1.44 9.69E-03 0.81 9.80E-01 2.05 6.42E-14
PA4385 groEL molecular chaperone GroEL 1.12 2.70E-01 2.12 1.78E-19 1.43 5.27E-10 4.11 3.33E-122
PA4386 groES co-chaperonin GroES 1.20 6.82E-02 2.55 6.71E-30 1.40 1.05E-08 3.88 1.48E-103
PA4760 dnaJ molecular chaperone DnaJ 1.21 4.61E-01 2.49 1.77E-05 3.89 8.0380E-17 8.22 1.01E-50
PA4761 dnaK molecular chaperone DnaK 1.12 4.35E-01 2.19 3.31E-11 1.16 9.73E-03 4.13 4.79E-61
PA4762 grpE heat shock protein GrpE 1.33 2.22E-01 3.19 8.51E-10 2.32 2.2053E-07 6.93 7.25E-45
PA5053 hslV ATP-dependent protease peptidase subunit 1.04 9.25E-01 2.71 8.79E-05 5.16 5.07E-18 11.90 6.68E-55
PA5054 hslU ATP-dependent protease ATP-binding subunit HslU 1.18 6.38E-01 2.95 9.95E-05 4.95 6.92E-14 11.30 1.22E-41
LPS O-ANTIGEN BIOSYNTHESIS
PA3149 wbpH glycosyltransferase WbpH 0.56 1.48E-02 0.16 2.37E-08 2.81 1.00E-14 2.98 8.4451E-17
PA3150 wbpG LPS biosynthesis protein WbpG 0.91 5.38E-01 0.15 2.46E-15 3.02 2.56E-30 2.84 9.6281E-28
PA3153 Wzx O-antigen translocase 0.96 8.49E-01 0.01 7.79E-09 2.13 5.49E-06 2.18 1.76E-06
PA3154 Wzy B-band O-antigen polymerase 1.24 4.39E-01 0.12 1.89E-05 3.89 4.96E-14 4.51 5.1553E-18
LPS PHOSPHORYLATION
PA5006 hypothetical protein 0.90 6.65E-01 1.01 9.93E-01 4.34 3.3053E-24 4.79 7.7100E-29
PA5007 wapG hypothetical protein 0.95 8.34E-01 0.81 4.72E-01 4.01 2.0919E-15 5.14 6.2265E-23
PA5008 wapP hypothetical protein 1.08 7.17E-01 0.83 3.52E-01 1.69 3.22E-05 2.08 9.1969E-09
PA5009 waaP lipopolysaccharide kinase WaaP 1.15 7.29E-01 0.76 5.12E-01 7.02 1.5556E-18 5.98 3.2275E-15
*

The fold change was calculated by dividing the mRNA level in each sample by the value of PAO1 incubated in HBSS alone (sample P0 in Table S3). The mRNA levels of each sample were shown in Table S3.