Table 1.

Lag period analysis of RT-QuIC reactions seeded with cerebral cortex from cases of sCJD and iCJD

	sCJD	hGH-iCJD		sCJD	hGH-iCJD	sCJD	hGH-iCJD	sCJD		hDM-iCJD	hGH-iCJD	hGH-iCJD
Patient subgroupa	All subtypes			VV2	VV2	MV2b	MV2 + i	MM1		MM1	MM1	MMi
Median lag periodc (h)	14.7	22.0		14.9	27.4	12.3	17.9	15.0		34.2	27.5	43.5
Number of patients	28	20		11	6	10	12	7		3	1	1
IQRd	10.6–17.1	16.6–27.5		11.2–19.0	17.3–38.5	9.2–16.5	16.3–24.5	10.7–20.5		19.3–62.0	–	–
Mann–Whitney teste	P ≤ 0.0001			P = 0.027		P = 0.007		P = 0.067			–	–
Kruskal–Wallis testf	–	–		P = 0.005						–	–	–

^aThe patients were separated into subgroups according to PRNP codon-129 genotype and PrP^res type

^bCases in the sCJD MV2 group were chosen to be representative of all sCJD cases with an MV PRNP codon-129 genotype that have type 2 PrP^res. Therefore, this group includes three type i + 2 cases, four type 2 cases and three type 1 + 2 cases

^cThe RT-QuIC period for each patient was calculated as the mean of at least four replicate analyses of one sample of cerebral cortex

^dIQR = Interquartile range

^eThe Mann–Whitney test was used to assess the statistical significance of the difference in the median lag periods in pairwise comparisons of patient subgroups on the basis that the shapes of the distributions of lag periods were similar for the patient subgroups

^fThe Kruskal–Wallis test was used for the combined analysis of sCJD (VV2, MV2, MM1) and hGH-iCJD (VV2, MV2) subgroups