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. 2016 Sep 5;36(10):1404–1416. doi: 10.1038/onc.2016.307

Figure 2.

Figure 2

Identification of DLX1 as a downstream target of FOXM1. (a) The qRT-PCR analysis showed that DLX1 expression was upregulated by 40-fold and 35-fold in the A2780cp cells, and by 50-fold and 40-fold in OVCA433 cells (**P<0.01, Student's t-test) after transient transfection of FOXM1B or FOXM1C, respectively. (b) The western blot analysis showed that both FOXM1B and FOXM1C upregulate DLX1 expression in OVCA433 and A2780cp cells. (c) The qRT-PCR analysis demonstrated a 20 and 40% reduction of DLX1 expression in the A2780cp (*P=0.03, Student t-test) and OVCA433 cells (*P=0.02, Student's t-test) following the depletion of endogenous FOXM1 by shRNA, respectively. (d) The western blot analysis showed that FOXM1 knockdown reduces DLX1 expression in the A2780cp and OVCA433 cells. The 18S RNA was used as an internal control for all qRT-PCR analyses.