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. 2016 Dec 22;13(2):467–474. doi: 10.3892/etm.2016.3996

Figure 4.

Figure 4.

PPARγ regulates VSMC calcification through activating Klotho. (A) The protein levels of Klotho in VSMCs treated with siRNA targeting Klotho as determined by western blot. Quantified protein levels of Klotho are shown in the right panel. β-actin was used as the loading control. **P<0.01 vs. control siRNA-transfected cells. (B) Calcium content in VSMCs subjected to Pi-induced calcification treated with RGZ or TZL (10 µM each), in the absence or presence of Klotho siRNA. (C) Pi uptake and (D) PiT-1 mRNA levels and (E) PiT-2 mRNA levels as determined by reverse-transcription quantitative polymerase chain reaction analysis in VSMCs subjected to Pi-induced calcification and treated with RGZ (10 µM), TZL (10 µM) or soluble Klotho (0.4 nM). Values are expressed as the mean ± standard error of the mean of three independent experiments. **P<0.05 vs. control siRNA-transfected cells treated with 1 mM Pi. #P<0.05, ##P<0.01 vs. control siRNA-transfected cells treated with 3 mM Pi. VSMC, vascular smooth muscle cell; Pi, inorganic phosphate; TZL, thiazolidinedione; RGZ, rosiglitazone; siRNA, small interfering RNA; PiT, Pi transporter.