Figure 3.

ASncmtRNA-2 is upregulated in HG-treated HRMCs. (A) HRMCs with the indicated treatments were harvested at 0, 8, 16 or 24 h. mRNA levels of ASncmtRNA-2 at each time point were recorded. (B) Relative ROS fluorescence levels were detected in HG and HRMCs with or without L-NAME treatment using dichloro-dihydro-fluorescein diacetate probes. (C) mRNA levels of TGFβ1 and FN were analyzed via RT-qPCR assay at 24 h post-treatment. *P<0.05 vs. LG; ^#P<0.05 vs. LG. ASncmtRNA-2, antisense mitochondrial non-coding RNA-2; ROS, reactive oxygen species; HG, high glucose; LG, low glucose; L-NAME, NG-nitro-L-Arginine methylester; HRMC, human renal mesangial cell; TGFβ1, transforming growth factor β1; FN, fibronectin; RT-PCR, revere transcription polymerase chain reaction.