Table 3.
Occludin and ZO-1 fluorescence quantification
| None | IL-1β | AdipoDren® | AdipoDren® + IL-1β | |
|---|---|---|---|---|
| HDLEC | ||||
| Occludin | 538,204 ± 189,997 | 61,057 ± 59,245* | 1,020,593 ± 134,129 | 655,570 ± 103,590## |
| ZO-1 | 1,986,154 ± 185,007 | 268,772 ± 77,869*** | 1,455,483 ± 66,005 | 1,186,348 ± 271,571## |
| HDBEC | ||||
| Occludin | 401,247 ± 21,304 | 108,283 ± 38,457*** | 262,024 ± 21,983* | 230,980 ± 39,389## |
| ZO-1 | 194,926 ± 44,295 | 73,387 ± 27,183* | 144,625 ± 4,250 | 162,937 ± 38,487# |
Cell fluorescence were measured using ImageJ software. Data are expressed as the corrected total cell fluorescence ± SD, calculated as: integrated density – (area of selected cell × mean fluorescence of background readings).
*
p < 0.05
**
p < 0.01 versus None
#
p < 0.05
#
p < 0.01 versus IL-1β.