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. 2017 Mar 14;10:144. doi: 10.1186/s13071-017-2077-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Amounts of total IgG1, IgG2 and IgE immunoglobulins differ between tick-resistant and tick-susceptible breeds of cattle. Amounts of total IgG1 (a), IgG2 (b) and IgE (c) immunoglobulins were measured in animals of a tick-susceptible (Holstein, blue squares) and a tick-resistant (Nelore, red open circles) breed of cattle before (baseline levels) and during three successive artificial infestations with ticks, when parasites were at the larval, nymphal and adult stages of their life-cycle. Dilutions of sera employed in indirect ELISAs were 1:100 and 1:5 for total IgG1 and IgG2, and for total IgE, respectively. Asterisks indicate the levels of significance between amounts of total IgG1, IgG2 and IgE immunoglobulins from Holstein and Nelore hosts and the specific statistical results are described in the text