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. 2017 Mar 14;16:42. doi: 10.1186/s12934-017-0657-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — ARF-TSS analysis of kstD transcripts. The translation initiation codon ATG, the TSS identified by ARF-TSS and the proposed −10 box appear in black. R1, R2 and F3 primers are indicated. A grey bar shows the sequence obtained by ARF-TSS from the circularized cDNA and the subsequent PCR amplification with R2 and F3