. 2017 Mar 14;17:65. doi: 10.1186/s12866-017-0975-9

Table 1.

Sequences of PCR primers targeting various targeting various virulent factor genes in this study

Target gene	Primer	Primer sequence (5′ – 3′)	Amplicon size (bp)	Reference
nheA	nheA 344 S	TACGCTAAGGAGGGGCA	480	[11]
nheA	nheA 843 A	GTTTTTATTGCTTCATCGGCT	480	[11]
nheB	nheB 1500 S	CTATCAGCACTTATGGCAG	754	[11]
nheB	nheB 2269 A	ACTCCTAGCGGTGTTCC	754	[11]
nheC	nheC 2820 S	CGGTAGTGATTGCTGGG	564	[11]
nheC	nheC 3401 A	CAGCATTCGTACTTGCCAA	564	[11]
hblA	HBLA1	GTGCAGATGTTGATGCCGAT	301	[11]
hblA	HBLA2	ATGCCACTGCGTGGACATAT	301	[11]
hblC	L2A	AATGGTCATCGGAACTCTAT	731	[11]
hblC	L2B	CTCGCTGTTCTGCTGTTAAT	731	[11]
hblD	L1A	AATCAAGAGCTGTCACGAAT	411	[11]
hblD	L1B	CACCAATTGACCATGCTAAT	411	[11]
cytK	CK-F-1859	ACAGATATCGG(GT)CAAAATGC	809	[46]
cytK	CK-R-2668	TCCAACCCAGTT(AT)(GC)CAGTTC	809	[46]
entFM	ENTA	ATGAAAAAAGTAATTTGCAGG	1, 269	[63]
entFM	ENTB	TTAGTATGCTTTTGTGTAACC	1, 269	[63]
Ces	cesF1	GGTGACACATTATCATATAAGGTG	1, 271	[16]
Ces	cesR2	GTAAGCGAACCTGTCTGTAACAACA	1, 271	[16]