Table 2.
Proteins of Fusobacterium nucleatum enhanced when incubated in cysteine-rich broth prior to protein extraction*
| Spot no. | Protein | Protein function |
|---|---|---|
| 1 | Glyceraldehyde-3-phosphate dehydrogenasea, b | Glycolytic protein |
| 2 | Bifunctional penicillin tolerance protein LytB/ribosomal protein S1 RpsAa | Translation |
| 3, 4 | Pyruvate kinaseb, c | Glycolytic protein |
| 5 | Recombination protein Ad /Histidyl-tRNA synthetasee, f | DNA repair/histidyl-tRNA aminoacylation |
| 6, 8 | Acetate kinaseb, g | Acetyl-CoA biosynthetic process |
| 7 | Electron transfer flavoprotein subunit alphab | Electron carrier activity |
| 9 | Phosphoglycerate kinaseb, h | Glycolytic protein |
| 10 | Zn-dependent alcohol dehydrogenase and related dehydrogenaseb | Oxidoreductase, zinc ion binding |
| 11 | Pyridoxal biosynthesis lyase PdxSb | Pyridoxal 5′-phosphate |
| 12 | Butyrate-acetoacetate CoA-transferase subunit Bb | Butyrate metabolism |
| 13 | Acetoacetate: butyrate/acetate coenzyme A transferaseb | Butyrate metabolism |
| 14 | Iron-sulfur cluster-binding proteinb | Iron and sulphur binding |
| 15 | (S)-2-hydroxy-acid oxidase chain Di /glycolate oxidase, subunit GlcDg | Oxidoreductase |
| 16, 17 | Rubrerythrina | Oxidoreductase, iron ion binding |
| 18 | PTS-system, N-acetylglucosamine-specific IIA componentb | Phosphotransferase system |
| 19 | Mannose-1-phosphate guanylyl transferase (GDP)b | GDP-mannose biosynthetic process, lipopolysaccharide biosynthetic process |
| 20 | Translation initiation inhibitorb | Deaminase activity |
| 21 | Anti-sigma F factor antagonistb | Regulation of transcription |
*Fusobacterium nucleatum OMGS 3938 was incubated in Todd Hewitt broth with and without cysteine. The spots that were enhanced when incubated in cysteine-rich broth were extracted for identification with LC- MS/MS
a Fusobacterium nucleatum subsp. polymorphum ATCC 10953
b Fusobacterium nucleatum subsp. nucleatum ATCC 25586
c Fusobacterium sp. 7_1
d Fusobacterium nucleatum subsp. nucleatum ATCC 23726
e Fusobacterium sp. 4_1_13
f Fusobacterium nucleatum ChDC F128
g Fusobacterium periodonticum ATCC 33693
h Desulfosporosinus sp. OT
i Fusobacterium nucelatum subsp. vicentii ATCC 49256