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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Nucl Med Biol. 2017 Jan 17;47:62–68. doi: 10.1016/j.nucmedbio.2017.01.004

Figure 1.

Figure 1

Biochemical analysis of anti-PSMA scFv-cys. (A) Schematic of anti-PSMA scFv-histidine-6-cysteine antibody structure and cDNA gene construction. (B) SDS gel electrophoresis of purified scFv-Cys under non-reducing (NR) and reducing (R) conditions and with coomassie staining. (C) Superdex 75 SEC analysis of purified scFv-cys antibody. (D) Immunoreactivity assay of purified 64Cu-DOTA-anti-PSMA-scFv-cys antibody (red) incubated with 20x molar excess of recombinant PSMA antigen (blue).