Figure 1. FIGURE 1: Localization of Cab5p.
(A) Equal amounts (20 mg) of the mitochondrial fraction (M) and post mitochondrial fraction (PMS) were resolved by SDS-PAGE and analyzed by immunoblotting with HA, PGK1 (cytosolic marker), PORIN (mitochondrial outer membrane marker) antibodies.
(B) Mitochondria were treated for 60 min at 4°C with proteinase K (prot K) (1 mg/ml). The filter was incubated with anti-HA, anti-CORE1, and anti-PORIN antibodies. Core1 was used as an inner membrane protein control.
(C) 150 µg of mitochondrial proteins were treated with TEGN buffer or TEGN and 0.1M NaCO3. The insoluble pellet (P) and supernatant (S) fractions were resolved by SDS-PAGE and analyzed by immunoblotting with HA and PORIN antibodies.