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. 2016 Dec 4;3(12):606–620. doi: 10.15698/mic2016.12.548

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Dityrosine fluorescence, as an indicator of sporulation, was examined after 3 days of sporulation on plates.

(B) Time course of meiotic nuclear divisions; the percentage of cells containing two or more nuclei is represented.

(C) Spore viability, as assessed by asci dissection, is presented. At least 144 spores were scored for each strain. Strains used in (A) are DP421 (wild type), DP994 (H4-K16R), DP1000 (H4-K16Q), DP422 (zip1), DP995 (zip1 H4-K16R) and DP1001 (zip1 H4-K16Q). Strains used in (B) and (C) are DP634 (wild type), DP635 (H4-K16R), DP636 (H4-K16Q), DP639 (zip1), DP640 (zip1 H4-K16R) and DP641 (zip1 H4-K16Q).