Figure 3. FIGURE 3: H4K16 acetylation is necessary for normal Mek1 and Hop1 phosphorylation.

Western blot analysis of Mek1 and Hop1 activation in wild type (DP421), zip1 (DP422), zip1 H4-K16Q (DP1001) and zip1 H4-K16R (DP995) strains throughout meiosis. Black arrows point the Mec1/Tel1-dependent phosphorylated form of Mek1, whereas white arrows mark the bands resulting from Mek1 autophosphorylation 21. Asterisks mark non-specific bands. H3T11 phosphorylation and Cdc5 inhibition were used as additional molecular markers for checkpoint activation. PGK was used as a loading control.