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Immunofluorescence of meiotic chromosome spreads from wild type (BR2495), sir2 (DP262), zip1 (DP1123) and zip1 sir2 (DP1124), stained with DAPI (red) as well as with anti-H4K16ac (green) and anti-Nsr1 (blue) antibodies. The arrows point to the rDNA region identified by Nsr1 staining. Representative nuclei are shown. Spreads were prepared after 16 h of meiotic induction for BR2495 and DP262 and 24 h for DP1123 and DP1124.
