(A) Transcriptome data of copper and GRISEA regulated
proteins. Colors and numbers indicate relative expression of transcripts
of the indicated genes. Relative expression was calculated by dividing
tpm of juvenile PQ-stressed samples by tpm of juvenile control (juv PQ),
middle-aged PQ-stressed samples by middle-aged control (ma PQ),
senescent PQ-stressed samples by senescent control (sen PQ), senescent
control by juvenile control (aging) and grisea by wild-type control
(grisea).
(B) Verification of transcriptome data of the selected genes
PaAox, PaSod2,
PaCtr1, PaCtr2, and
PaCtr3. Results from SuperSAGE analysis are shown
in tpm (tags per million). The results of the corresponding qRT-PCR are
depicted as relative expression levels to the reference genes
PaPorin and PaRpl19. Data are
displayed as mean ± standard error. n = 3. (C) Measurement
of complex IV and PaAOX activity in control and PQ-stressed mycelia.
Juvenile P. anserina was grown as described for
transcriptome analysis. The oxygen consumption of 2 to 9 mg mycelium was
measured in a high resolution respirometer. To determine the percentage
of complex IV and PaAOX dependent respiration, a final concentration of
4 mM SHAM and 1 mM KCN was used to inhibit PaAOX and complex IV,
respectively. Bars are mean ± standard error of 6 - 7 experiments with
two different cultures. n = 20. Student’s t-test: pV = 0.0019.