Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 6;1(1):9–20. doi: 10.15698/mic2014.01.120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial No Derivatives License, which permits the copy and distribution of the material in any medium or format as well as to remix transform, and build upon the material, provided the original work is properly cited and the material not used for commercial purposes. If the material is remixed, transformed or build upon, the modified material can only be distributed under the same license as the original.

PMC Copyright notice

Differentiation of BSF YFP-ATG8.2 ATG5 RNAi mutants (48 h after tet induction) was stimulated by switching cells to differentiation media and incubating at 27^oC. Cells were analysed for characteristic PCF markers such as EP-procyclin expression and nuclear/kinetoplast re-configuration.

(A) Western blot of whole cell extracts taken at specific times after induction of differentiation and probed with EP-procyclin, anti-EF1α and anti-OPB.

(B) Fluorescent microscopy of cells 24 h post induction, fixed and labelled with anti-EP procyclin (red) and DAPI (blue) to show changes in the nuclear configuration (nucleus, N and kinetoplast, K). Scale bar 5 µm.