Table 1.

Main features of N. crassa alternative NAD(P)H dehydrogenases.

^a Ca²⁺ stimulates the oxidation of cytosolic NADH in a Δnde-1Δnde-2 double mutant, but not in the triple mutant Δnde-1Δnde-2Δndi-1, indicating that NDI-1 may be stimulated by Ca²⁺ 18. NA: not assessed.

Protein	Topology	Substrate specificity	Ca2+	pH	Reference(s)
NDE-1	External	Cytosolic NADPH	Ca2+-dependent	Physiological pH	16,17
NDE-2	External	Cytosolic NADH and NADPH	-	NADH throughout the pH range and NADPH at acidic pH	18
NDE-3	External	Cytosolic NADH and NADPH	-	NA	19
NDI-1	Internal	Matrix NADH	Ca2+-stimulated? a	NA	15,18